ABSTRACT
Objective To study the chemical constituents of Ophiorrhiza rosea.Methods The chemical constituents in O.rosea were extracted with 90% methanol,isolated and purified by column chromatography on silica gel,alumina gel,MCI gel,and macroporous resin adsorption.All the compounds were identified based on spectral analyses(MS,1H-NMR,and 13C-NMR).Results Eleven compounds were isolated from O.rosea.They were characterized as Harman(Ⅰ),2-hydroxy-3-hydroxymethylanthraquinone(Ⅱ),1hydroxy-2-hydroxymethylanthraquinone-3-O-?-D-glucoside(Ⅲ),1-hydroxy-2-hydroxymethylanthraquinone-3-O-?-D-primeveroside(Ⅳ),ursolic acid(Ⅴ),3?,19,24trihydroxy-12-ursen-28-oic acid(Ⅵ),19,23-dihydroxy-3-oxo-12-ursen28-oic acid(Ⅶ),3?,24-dihydroxy-12-oleanen-28-oic acid(Ⅷ),3,20-epoxy-3?,16-dihydroxy-15-oxo-7-pimaren-19,6-olide(Ⅸ),?-stigmasterol-3-O-?-D-glucoside(Ⅹ),and ?-sitosterol(Ⅺ).Conclusion This is the first report on the chemical constituents of O.rosea to find that it contains plentiful alkaloid Harman,multiform of triterpenes and anthraquinones.
ABSTRACT
Objective To study the chemical constituents in the roots of Flemingia macrophylla Methods To separate and purify compounds by column chromatography and TLC, and to determine their chemical structures by their physical characters and spectral data. Results Eleven compounds were purified from the extraction in the roots of F. macrophylla, among them four are isoflavones, three are flavanones, and one is flavanol. They are genistein (Ⅰ), orobol (Ⅱ), 5, 7, 4′-trihydroxyisoflavone-7-O-?-D-glucopyranoside (Ⅲ), 5, 7, 4′-trihydroxy-8, 3′-diprenylflavanone (Ⅳ), 5, 7, 4′-trihydroxy-6-prenylisoflavone (Ⅴ), flemichin D (Ⅵ), lespedezaflavanone A(Ⅶ), ouratea-catechin (Ⅷ), 3, 4, 5-trimethoxybenzene-O-?-D-glucopyranoside (Ⅸ), stigmasterol-3-O-?-D-glucopyranoside (Ⅹ), and stigmasterol (Ⅺ). Conclusion Compounds Ⅲ—Ⅴ, and Ⅶ—Ⅺ are found from this plant for the first time. All the compounds are found from the roots of this plant for the first time. The active components, genistein and its isoflavone analogs, are main constituents in the roots of F. macrophylla
ABSTRACT
Objective To investigate the chemical constituents in the leaves of Dracaena cochinchinensis.Methods The compounds were separated with column chromatography and their chemical structures were identified by physicochemical and spectral method,respectively.Results Thirteen compounds were isolated from the plant. They were identified as isorhamnerin(Ⅰ),quercetin(Ⅱ),25(R)-spirostane-5-en-3?-ol(Ⅲ),gracillin(Ⅳ),25(R)-spirostane-5-en-3?,14?-diol(Ⅴ),25(R)-spirostane-5-en-3?,14?-diol-3-O-?-D-glucopyranoside(Ⅵ),25(R)-spirostane-5-en-3?,14?-diol-3-O-?-L-rhamnopyranosy(1→4)-?-D-glucopyranoside(Ⅶ),25(R)-spirostane-14?-hydroxy-4-en-3-one(Ⅷ),7?-hydroxysistosterol-3-O-?-D-glucopyranoside(Ⅸ),?-stigmasterol(Ⅹ),stigmasterol-3-O-?-D-glucopyranoside(Ⅺ),daucosterol (Ⅹ Ⅱ),and methyl ?D-glucopyranoside(Ⅹ Ⅲ).Conclusion Spirostane-type steroids are the major constituents in the leaves of D.cochinchinensis.Compounds Ⅰ-Ⅲ,Ⅴ,Ⅵ,Ⅷ,and Ⅸ are isolated from this plant for the first time.
ABSTRACT
Objective The experiment compared the chemical components in the roots,stems,and leaves of Dracaena cochinchinensis with those in the raw material of Dragon's blood,in order to find the possible raw material of Dragon's blood.Methods Technology of thin layer chromatography(TLC) and high performance liquid chromatography(HPLC).Results The results showed that the roots,stems,and leaves of D.cochinchinensis just contained small amount of loureirin A,the contents of loureirin A of the three samples are: 58,53,and 15 ?g/g,respectively.Loureirin B was only detected in the leaves with content of 2 ?g/g.Conclusion From the analysis of peak areas and the retention time,there are only slight correlations among the components of the four samples and the chemical components among the roots,stem,and leaves are different.Moreover,there is too low content of loureirins in the roots,stem,and leaves to be raw material of Dragon's blood.